Biology II

Chapter 19 Objectives

1. Explain how advances in recombinant DNA technology have helped scientist study the eukaryotic genome.

2. Describe the natural function of restricion enzymes.

3. Describe how restriction enzymes and gel electroghories are used to isolate DNA fragments.

4. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA strand.

5. Outline the procedures for producing plasmid and phage vectors.

6. Explain how vectors are used in recombinant DNA technology.

7. List and describe the two major sources of genes for cloning.

8. Describe the function of reverse transcriptase in retroviruses and explain how they are useful in recombinant DNA technology.

9. Describe how “genes of interest” canbe identified with the use of a probe.

10. Explain the importance of DNA synthesis and sequencing to modern studies of eukaryotic genomes.

11. Outline the Sanger method for sequencing DNA.

12. Describe how bacteria can be induced to produce eukaryotic gene products

13. List some advantages for using yeast in the production of gene products

14. Describe some practical applications of recombinant DNA technology in biolocial research

15. List and describe four complementary approaches used to map the human genome

16. Explain how RFLP analysis and PCR can be applied to the human genome project

17. Describe how recomninant DNA technology can have medical appliactions such as the diagonisis genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products

18. Describe how gene manipulation has practical applications for agricultural

19. Desribe how plant genes can be manipulated using the Ti plasmid carried by Agrobacterium as a vector

20. Explain how foreign DNA made be transfered into monocotyledonous plants

21. Describe how recombinant DNA studies and the biotechnical industry are regulated with regards to safety and policy matters